|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Suínos e Aves. |
Data corrente: |
13/11/2018 |
Data da última atualização: |
03/01/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
OKINO, C. H.; IBELLI, A. M. G.; BRENTANO, L.; ZANELLA, R.; MARQUES, M. G. |
Afiliação: |
CINTIA HIROMI OKINO, CPPSE; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; LIANA BRENTANO, CNPSA; Ricardo Zanella, Universidade de Passo Fundo; MARIANA GROKE MARQUES, CNPSA. |
Título: |
Evaluation of candidate reference genes for investigation of the uterine tissue and corpus luteum of pigs on day 6 after artificial insemination. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Animal Reproduction Science, v. 198, p. 202-209, 2018. |
DOI: |
10.1016/j.anireprosci.2018.09.020 |
Idioma: |
Inglês |
Conteúdo: |
Housekeeping genes (HKG) are genes necessary for the maintenance of basal cellular functions, regardless of the specific roles within a tissue. It, therefore, is expected that these genes will maintain a relatively constant expression profile when there are varying physiological conditions. The identification of tissue specific reference genes is highly important for the normalization of gene expression profiles among different tissues. In this sow study, the objective was to identify stable reference genes in the uterine tissue and corpus luteum (CL), 6 days post-artificial insemination. The stability of ubiquitin (UBB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), DNA topoisomerase 2-beta (TOP2B), histone H3 (H3F3A) and hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) abundances of mRNA were evaluated using the Bestkeeper technique. Briefly, total RNA was extracted for each tissue from 20 gilts (n=20), processed by RT-qPCR and submitted to analysis using the Bestkeeper technique, which allowed for the evaluation of the consistency in abundance of mRNA for the reference genes. For all evaluated genes, the abundance of mRNA was relatively consistent in the uterine tissue, with the greatest abundance being for the GAPDH and TOP2B genes. The analysis of these genes in the CL, however, indicated there was a relatively greater variation of mRNA abundance for the various reference genes. Data suggest that UBB was the reference gene with the most consistent relative abundance of mRNA and that this gene could be used as a reference for corpora lutea analyses of mRNA MenosHousekeeping genes (HKG) are genes necessary for the maintenance of basal cellular functions, regardless of the specific roles within a tissue. It, therefore, is expected that these genes will maintain a relatively constant expression profile when there are varying physiological conditions. The identification of tissue specific reference genes is highly important for the normalization of gene expression profiles among different tissues. In this sow study, the objective was to identify stable reference genes in the uterine tissue and corpus luteum (CL), 6 days post-artificial insemination. The stability of ubiquitin (UBB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), DNA topoisomerase 2-beta (TOP2B), histone H3 (H3F3A) and hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) abundances of mRNA were evaluated using the Bestkeeper technique. Briefly, total RNA was extracted for each tissue from 20 gilts (n=20), processed by RT-qPCR and submitted to analysis using the Bestkeeper technique, which allowed for the evaluation of the consistency in abundance of mRNA for the reference genes. For all evaluated genes, the abundance of mRNA was relatively consistent in the uterine tissue, with the greatest abundance being for the GAPDH and TOP2B genes. The analysis of these genes in the CL, however, indicated there was a relatively greater variation of mRNA abundance for the various reference genes. Data suggest that UBB was the reference gene with the most consistent relative a... Mostrar Tudo |
Palavras-Chave: |
GAPDH; Housekeeping; TOP2B. |
Thesagro: |
Inseminação; Reprodução Animal; Suíno; Útero. |
Thesaurus Nal: |
Gene expression; Sows; Ubiquitin. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02483naa a2200301 a 4500 001 2099280 005 2019-01-03 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1016/j.anireprosci.2018.09.020$2DOI 100 1 $aOKINO, C. H. 245 $aEvaluation of candidate reference genes for investigation of the uterine tissue and corpus luteum of pigs on day 6 after artificial insemination.$h[electronic resource] 260 $c2018 520 $aHousekeeping genes (HKG) are genes necessary for the maintenance of basal cellular functions, regardless of the specific roles within a tissue. It, therefore, is expected that these genes will maintain a relatively constant expression profile when there are varying physiological conditions. The identification of tissue specific reference genes is highly important for the normalization of gene expression profiles among different tissues. In this sow study, the objective was to identify stable reference genes in the uterine tissue and corpus luteum (CL), 6 days post-artificial insemination. The stability of ubiquitin (UBB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), DNA topoisomerase 2-beta (TOP2B), histone H3 (H3F3A) and hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) abundances of mRNA were evaluated using the Bestkeeper technique. Briefly, total RNA was extracted for each tissue from 20 gilts (n=20), processed by RT-qPCR and submitted to analysis using the Bestkeeper technique, which allowed for the evaluation of the consistency in abundance of mRNA for the reference genes. For all evaluated genes, the abundance of mRNA was relatively consistent in the uterine tissue, with the greatest abundance being for the GAPDH and TOP2B genes. The analysis of these genes in the CL, however, indicated there was a relatively greater variation of mRNA abundance for the various reference genes. Data suggest that UBB was the reference gene with the most consistent relative abundance of mRNA and that this gene could be used as a reference for corpora lutea analyses of mRNA 650 $aGene expression 650 $aSows 650 $aUbiquitin 650 $aInseminação 650 $aReprodução Animal 650 $aSuíno 650 $aÚtero 653 $aGAPDH 653 $aHousekeeping 653 $aTOP2B 700 1 $aIBELLI, A. M. G. 700 1 $aBRENTANO, L. 700 1 $aZANELLA, R. 700 1 $aMARQUES, M. G. 773 $tAnimal Reproduction Science$gv. 198, p. 202-209, 2018.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 240 | |
6. | | PEIXOTO, J. de O.; IBELLI, A. M. G.; CANTAO, M. E.; LEDUR, M. C. Condronecrose bacteriana com osteomielite (BCO): um problema subestimado na avicultura de corte. Guia Gessulli da Avicultura e Suinocultura Industrial, Itu, ed. 266, ano 38, n. 5, p. 24-30, 2015.Tipo: Artigo de Divulgação na Mídia |
Biblioteca(s): Embrapa Suínos e Aves. |
| |
8. | | IBELLI, A. M. G.; REGITANO, L. C. de A.; NICIURA, S. C. M. Extração de RNA. In: REGITANO, L. C. de A.; NICIURA, S. C. M.; IBELLI, A. M. G.; GOUVEIA, J. J. de S. Protocolos em biologia molecular aplicada à produção animal. São Carlos: Embrapa Pecuária Sudeste, 2007. p. 9-11.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
17. | | GAVA, D.; IBELLI, A. M. G.; SCHAEFER, R.; ZANELLA, J. R. C. Multiplex PCR for simultaneous detection of five single-stranded swine DNA viruses. In: Journal of the Brazilian Society for Virology, Ribeirão Preto, v. 18, p. 215-216, 2013. Suppl. 1. Edição dos anais do 24º. Brazilian Congress of Virology e 8º. Mercosur Meeting of Virology, Porto Seguro, set. 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Suínos e Aves. |
| |
Registros recuperados : 240 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|